Modeling human adaptive immune responses with tonsil organoids (Publications)
Most of what we know about adaptive immunity has come from inbred mouse studies, using methods that are often difficult or impossible to confirm in humans. In addition, vaccine responses in mice are o
Case-control study of individuals with discrepant nucleocapsid and spike protein SARS-CoV-2 IgG results (Publications)
BACKGROUND: Laboratory-based methods for SARS-CoV-2 antibody detection vary widely in performance. However, there are limited prospectively-collected data on assay performance, and minimal clinical in
Shared B cell memory to coronaviruses and other pathogens varies in human age groups and tissues (Publications)
Vaccination and infection promote the formation, tissue distribution, and clonal evolution of B cells, which encode humoral immune memory. We evaluated pediatric and adult blood and deceased adult org
Plasma as an alternative COVID-19 diagnostic specimen in a hospitalized patient negative for SARS-CoV-2 by nasopharyngeal swab (Publications)
We present the case of an inpatient with pneumonia and repeatedly negative nasopharyngeal SARS-CoV-2 testing. In such challenging cases, alternative diagnostic options include lower respiratory tract
Adjuvanting a subunit COVID-19 vaccine to induce protective immunity (Publications)
The development of a portfolio of COVID-19 vaccines to vaccinate the global population remains an urgent public health imperative(1). Here we demonstrate the capacity of a subunit vaccine, comprising
Increased viral variants in children and young adults with impaired humoral immunity and persistent SARS-CoV-2 infection: a consecutive case series (Publications)
BACKGROUND: There is increasing concern that persistent infection of SARS-CoV-2 within immunocompromised hosts could serve as a reservoir for mutation accumulation and subsequent emergence of novel st
Evaluation of SARS-CoV-2 total antibody detection via a lateral flow nanoparticle fluorescence immunoassay (Publications)
BACKGROUND: The coronavirus disease 2019 (COVID-19) endgame may benefit from simple, accurate antibody testing to characterize seroprevalence and immunization coverage. OBJECTIVES: To evaluate the per
Efficient identification of high-titer anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody plasma samples by pooling method (Publications)
CONTEXT.-: The ongoing COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has elicited a surge in demand for serologic testing to identify previously infected
Use of outpatient-derived COVID-19 convalescent plasma in COVID-19 patients before seroconversion (Publications)
BACKGROUND: Transfusion of COVID-19 convalescent plasma (CCP) containing high titers of anti-SARS-CoV-2 antibodies serves as therapy for COVID-19 patients. Transfusions early during disease course was
SARS-CoV-2 nucleocapsid plasma antigen for diagnosis and monitoring of COVID-19 (Publications)
BACKGROUND: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen in blood has been described, but the diagnostic and prognostic role of antigenemia is not wel